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Rat Air Pouch Model of Inflammation. Air pouches were produced by subcutaneous injection of 20 ml sterile air into the intrascapular area of the back of male Lewis rats 175200 g ; Charles River Laboratories, Inc., Wilmington, MA ; , six animals per dose group. Pouches were allowed to develop for 1 day. Animals were fasted with free access to water. Compounds or vehicle were administered by gavage 2 h prior to injection of 2 ml suspension of carrageenan Sigma-Aldrich ; dissolved in saline into the pouch. At 3 h postcarrageenan injection, the pouch fluid was collected by lavage with 1 ml of cold heparin-saline. The fluid was centrifuged at 800g for 10 min at 4C, and the supernatants were collected for analysis of PGE2 by ELISA. At the end of the 3-h postcarrageenan injection, rats were anesthetized with a CO2 O2 gas, and blood was collected by heart stick. The gastrointestinal tract was exposed and observed for lesions. Sections of stomach mucosa were dissected and immediately frozen for further prostaglandin analysis. Frozen tissues were processed by homogenization in 70% ethanol. After centrifugation, the supernatants were collected, dried under a stream of nitrogen, and resuspended in ELISA buffer for PGE2 determination by ELISA. Acute Carrageenan-Induced Edema and Hyperalgesia in the Rat. Paw edema was induced by injecting 0.1 ml of a 1% carrageenan saline solution into the hind paw of male Sprague-Dawley rats 180 280 g ; , six animals per dose group. Prior to experiments, the rats were allowed free access to food and water. Compounds or vehicle 0.5% methylcellulose in water ; were administered orally 1 h before the carrageenan injection, and paw volume was measured by water displacement at intervals thereafter. Analgesic activity was assessed as inhibition of hyperalgesia produced in response to a thermal stimulus from a radiant heat source high-intensity projector lamp bulb ; positioned under a Plexiglas floor directly beneath the hind paw. The withdrawal latency of the affected paw was compared with the contralateral i.e., normal ; paw and determined to the nearest 0.1 s with an electronic clock circuit and a microcomputer. Each point represents either the change in paw volume or withdrawal. Rat Adjuvant Arthritis Model. Dose-response curves for antiinflammatory activity were established as described by Billingham 1983 ; . Male Lewis rats 180 200 g ; , six animals per dose group, were injected with 1 mg of heat-killed mycobacterium butyricum Difco, Detroit, MI ; suspended in light mineral oil 1 mg 0.025 ml ; [subcutaneously in the plantar surface of the right hind paw day 0 ; ]. Viropharma commercializes vancocin r ; , approved for oral administration for treatment of antibiotic- associated pseudomembranous colitis caused by clostridium difficile and enterocolitis caused by staphylococcus aureus, including methicillin-resistant strains for prescribing information, please download the package insert at site viropharma currently focuses its drug development activities in viral diseases including cytomegalovirus cmv ; and hepatitis c hcv and amoxil.
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Abstract 1660 DEVELOPMENT AND VALIDATION OF A QUALITY OF LIFE MODULE SPECIFIC TO HAIR PROBLEMS IN MENOPAUSAL WOMEN Linda Abetz, Marci Plante, Constance McLaughlin-Miley, Elise Olsen, David Gash, Deborah Arbit, Mapi Values, Bollington, Cheshire, UK The purpose of this study was to develop a validated questionnaire to measure the extent and impact of hair changes on the quality of life of menopausal women. In-depth interviews were conducted with 45 menopausal women and 25 clinicians in the US, English and French Canada to ascertain womens experiences and clinicians treatment decisions regarding hair problems. Based on the results, the Hair Assessment Instrument HAIr ; was developed. Subsequently, the HAIr was administered to 10 women in each country to assess feasibility, coherence and comprehensiveness. Finally, 200 women in the US and English Canada were asked to complete the HAIr and psychometric testing was performed. Based on the pilot test, psychometric validation, and clinician input, the HAIr was shortened. Interviews provided information about different types of hair problems, hair product use, and the impact of hair problems on patient well-being, yielding a questionnaire of 50 items. Psychometric validation resulted in a shortened questionnaire comprised of 34 items with 8 domains Hair Quality, Hair Quantity, Hair Texture Strength, Dandruff, Fingernails, Emotional Impact, Changes in Hair Quality, and Changes in Hair Quantity ; . The HAIr demonstrated acceptable reliability alpha 0.70; intra-class correlation coefficient 0.70 ; and known groups validity women recruited with hair problems had significantly poorer scores than those women recruited without hair problems ; for the majority of scales. The HAIr is appropriate for assessment of hair problems and the impact of those hair problems on menopausal women. Future research will assess the HAIrs sensitivity to clinical changes over time and atrovent and altace, for instance, altace interactions!

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Synopsis Officials have announced that the mystery sever acute respiratory syndrome SARS ; that has been blamed for 18 deaths worldwide may be responsible for up to 31 deaths in China, where it is believed that the current outbreak originated. If the link is confirmed, the global death toll will have increase to at least 49. According to figures release by the World Health Organisation almost 500 people worldwide have been affected. Countries with reported cases so far include, Canada, China including Hong Kong and Taiwan ; , France, Germany, Italy, Republic of Ireland, Singapore, Spain, Switzerland, Thailand, United Kingdom, United States and Vietnam Title Source Link How contagious are common respiratory tract infections? - A review N Engl J Med 2003; 348: 1256-1266 : content.nejm cgi content full 348 13 1256 subscribers only. North America: United States: Veterans Affairs Medical Center and Creighton University, Omaha, NE: R.J. Anderson, MD; Oregon Health Sciences University, Portland, OR: S. Anderson, MD; Research Institute of Dallas, Dallas, TX: S. Aronoff, MD; Cedars Sinai Medical Center, Los Angeles, CA: P. Barnett, MD; University of Colorado Health Science Center, Denver, CO: T. Berl, MD; Radiant Research of Phoenix, Phoenix, AZ: M. Block, MD, L. Nelson, MD; Medical College of Wisconsin, Milwaukee, WI: S. Blumenthal, MD, B. Bresnahan, MD; Renal Research Baystate Medical Center, Springfield, MA: G.L. Braden, MD, E.M. Benjamin, MD, M.H. O'Shea, MD; Vanderbilt University Medical Center, Nashville, TN: J. Lewis, MD, G. Schulman, MD; Thomas Jefferson University, Philadelphia, PA: J.F. Burke, Jr., MD, K. Sharma, MD; Charlotte Clinical Research, Charlotte, NC: G. Collins, MD; Louisville Metabolic & Atherosclerosis Research Center, Louisville, KY: J. Cyrus, MD; Maricopa Medical Center, Phoenix, AZ: W. Dachman, MD; Diabetes Center of Western New York, Buffalo, NY: P. Dandona, MD; Marshfield Clinic, Marshfield, WI: R.A. Dart, MD; Veterans Administration Medical Center, Northport, NY: T. Dixon, MD; Beaumont Nutritional Clinic, Birmingham, MI: M. Doyle, MD; Theodore Duncan & Associates, Philadelphia, PA: T. Duncan, MD; Rhode Island Hospital, Providence, RI: L. Dworkin, MD; Diabetes, Endocrinology & Metabolic Disorders, San Diego, CA: D. Einhorn, MD; Washington Hospital Center, Washington, DC: G. Eisner, MD, J. Moore Jr, MD; University Hospital Clinic Ohio State University, Columbus, OH: M. Falkenhain, MD; Cardiovascular Research Center of South Florida, Miami, FL: J. Fialkow, MD; Hill Top Research, Portland, OR: P. Fisher, MD; Veterans Administration Medical Center, Spokane, WA: E. Fishman, MD; New York Medical College and Nephrology Associates of Westchester, Hawthorne, NY: R. Garrick, MD; Veterans Administration Hospital, Tucson, AZ: S. Goldman, MD.

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