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Nomas have been found to secrete gonadotropin subunit or Sanno et al. 1992, 1996d ; . Experimental models of pituitary adenomas have included estrogeninduced PRL-secreting adenomas and TSH-secreting adenomas induced by thyroidectomy. Recently, genetic engineering techniques have produced functioning pituitary adenomas, e.g., GH-secreting adenomas in human growth hormone-releasing hormone hGHRH ; transgenic mice. In addition, the recent development of specific gonadotropin subunit expression in SV40 promoter transformation and subsequent immortalization with established cell lines have contributed to clarification of transcriptional mechanisms Windle et al. 1990 ; . This review focuses the contribution of IHC and ISH to our understanding of human and experimental pituitary adenomas, i.e. a ; functional classification, b ; transcriptional mechanisms, and c ; secretory processes.
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